Movement Characteristics of Bovine Epididymal Spermatozoa: Effects of Forward Motility Protein and Epididymal Maturation
نویسندگان
چکیده
منابع مشابه
Adenine nucleotide mediation of fructolysis and motility in bovine epididymal spermatozoa.
Fructolysis is stimulated 3to 5-fold in bovine epididymal spermatozoa when the latter are preincubated with caffeine, theophylline, cyclic adenosine 3’, 5’-monophosphate (CAMP), and N6, 02’-dibutyryl adenosine 3’,5’-monophosphate’((Bt)2CAMP). No increase in fructolysis is observed if phosphodiesterase inhibitors or cyclic nucleotides are added to freshly washed cells or to preincubated cells ju...
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Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin bl...
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Acquisition of fertilization ability by spermatozoa during epididymal transit occurs in part by the transfer of molecules from membranous vesicles called epididymosomes. Epididymosomes are heterogeneous in terms of both size and molecular composition. Exosomes and other related small membranous vesicles (30-120 nm) containing tetraspanin proteins on their surface are found in many biological fl...
متن کاملProteomic changes in mammalian spermatozoa during epididymal maturation.
Epididymal maturation is associated with the activation of a cAMP-induced tyrosine phosphorylation cascade, which is ultimately associated with the expression of capacitation-dependent sperm functions, such as hyperactivated movement and acrosomal exocytosis. As spermatozoa progress through the epididymis they first acquire the capacity to phosphorylate tyrosine on targets on the principal piec...
متن کاملChanges in calmodulin level and cAMP-dependent protein kinase activity during epididymal maturation of ram spermatozoa.
Calmodulin concentration and cAMP-dependent protein kinase activity were simultaneously determined on ram spermatozoa collected by cannulation of successive segments of the epididymal tubule. Epididymal transit was characterized on one hand by an overall decrease in the calmodulin level and on the other by a dramatic rise in the cAMP-dependent protein kinase activity. In contrast to the calmodu...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1983
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod29.2.389